《植物生理学报》 2010, 46(1): 33-36

珠美海棠试管苗的土壤支撑生根培养和带坨移栽

柴慈江*, 卢兴霞, 苏卫国, 裴君, 王玉

天津农学院园艺系, 天津300384

通信作者：柴慈江；E-mail: cijiang336@hotmail.com；Tel: 022-81785 221

摘　要：

以粘壤土做培养基支撑物, 选用只含有0.5 mg•L-1 IBA和15 g•L-1蔗糖的液体培养基, 珠美海棠试管苗茎段生根培养的 结果显示, 试管苗生根率可达到100.0%, 明显高于琼脂支撑培养, 根长也显著提高, 并且长出正常的根毛。土支撑培养生根 的珠美海棠试管苗开瓶炼苗 21 d 后带坨移入营养钵中, 移栽后不喷雾、并毋需覆盖塑膜、午后空气相对湿度控制在50%~65%, 其成活率达到 92.2%; 在开瓶炼苗 21 d 后, 黑暗中叶片气孔的关闭率从 26.7% 增加到 88.5%。

关键词：珠美海棠; 试管苗; 土支撑; 生根; 带坨移栽

收稿：2009-10-19   修定：2009-11-15

资助：国家科技部星火计划(2008GA610015)和天津市科委项目(08ZHXHNC07000)。

Rooting in Soil Supporting Medium and Transplanting with Lump of Malus zumi (Matsumura) Rehd Plantlet in vitro

CHAI Ci-Jiang*, LU Xing-Xia, SU Wei-Guo, PEI Jun, WANG Yu

Department of Horticulture, Tianjin Agricultural College, Tianjin 300384, China

Corresponding author: CHAI Ci-Jiang; E-mail: cijiang336@hotmail.com; Tel: 022-81785 221

Abstract:

Stem sections from the Malus zumi plantlet were cultured in vitro in lipid medium with clay loam soil supporting, and the medium contained 0.5 mg·L^-1 IBA and 15 g·L^-1 sugar addition. The rooting rate was 100.0%, which was obviously higher than that of the plantlets cultured in agar supporting medium. The plantlets cultured in soil supporting medium had root hairs and the roots were obviously longer than those of the plantlets cultured in agar medium. The rooted plantlets cultured in soil supporting medium were acclimatized in opened bottles for 21 days, and then were transplanted with lump into soil. Without spraying water or covering plastic film, and under relatively low air humidity from 50% to 65% in the afternoon, 92.2% of the plantlets were survived. Observation the stomata of the M. zumi plantlets in vitro showed that the stomata closing rate of the plantlets in dark condition increased from 26.7% to 88.5% after acclimatized in opened bottle for 21 days.

Key words: Malus zumi; plantlet in vitro; soil supporting; rooting; transplanting with lump